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A CRISPR/Cas9 toolkit for multiplex genome editing in plants

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Synthetic biology - Wikipedia

Metrics details. This toolkit requires no restriction enzymes besides Bsa I to generate final constructs harboring maize-codon optimized Cas9 and one or more gRNAs with high efficiency in as little as one cloning step. The toolkit was validated using maize protoplasts, transgenic maize lines, and transgenic Arabidopsis lines and was shown to exhibit high efficiency and specificity. More importantly, using this toolkit, targeted mutations of three Arabidopsis genes were detected in transgenic seedlings of the T1 generation. Moreover, the multiple-gene mutations could be inherited by the next generation.
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How do mycorrhizae work?

Have you ever wondered how plants are able to pull water out of the ground? It relies on some pretty basic physical principles operating within unique plant structures, and anyone can understand it. Xylem is composed of long, hollow tubes formed by overlapping cells. As these cells grow, they stretch out and elongate, die, and leave behind hollow cavities that are all interconnected to form one long tube. Plants contain many xylem vessels stretching from the roots to the tips of the leaves, just like a series of drinking straws.
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